Device

Part:BBa_K1377001:Design

Designed by: Jade Minseob Yoo   Group: iGEM14_Korea_U_Seoul   (2014-10-09)


spaA backward + sortase A + spaB + spaC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 4073
    Illegal EcoRI site found at 4250
    Illegal EcoRI site found at 6690
    Illegal EcoRI site found at 6777
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1642
    Illegal PstI site found at 3331
    Illegal PstI site found at 3649
    Illegal PstI site found at 7735
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 4073
    Illegal EcoRI site found at 4250
    Illegal EcoRI site found at 6690
    Illegal EcoRI site found at 6777
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1642
    Illegal PstI site found at 3331
    Illegal PstI site found at 3649
    Illegal PstI site found at 7735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 4073
    Illegal EcoRI site found at 4250
    Illegal EcoRI site found at 6690
    Illegal EcoRI site found at 6777
    Illegal EcoRI site found at 6879
    Illegal BamHI site found at 97
    Illegal BamHI site found at 5858
    Illegal XhoI site found at 365
    Illegal XhoI site found at 1073
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 4073
    Illegal EcoRI site found at 4250
    Illegal EcoRI site found at 6690
    Illegal EcoRI site found at 6777
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1642
    Illegal PstI site found at 3331
    Illegal PstI site found at 3649
    Illegal PstI site found at 7735
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 4073
    Illegal EcoRI site found at 4250
    Illegal EcoRI site found at 6690
    Illegal EcoRI site found at 6777
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1642
    Illegal PstI site found at 3331
    Illegal PstI site found at 3649
    Illegal PstI site found at 7735
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 4458
    Illegal NgoMIV site found at 7397
    Illegal AgeI site found at 4941
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3432


Design Notes

Just include all cluster that related to make pili A And there is no good restriction enzyme unique cut site, so we slice the cluster to two


Source

Genomic DNA of Corynebacterium diphtheriae

References